ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of water extracts from Duhuo Jisheng decoction on chondrocyte G1 phase.</p><p><b>METHODS</b>Chondrocytes were collected from four-week-old SD rats to establish the chondrocyte in vitro culture system. The third generation of chondrocytes was intervened. MTT method was used to measure the effect of water extracts from different concentrations of Duhuo Jisheng decoction on chondrocyte activity. The expressions of Chondrocyte Cyclin D1, CDK4, CDK6 and P21 mRNA in the blank group and low, middle and high-dose groups (100, 200, 400 mg x L(-1)) were detected by RT-PCR method.</p><p><b>RESULT</b>The MTT assay showed that the chondrocyte activity significantly increased within specific drug concentrations (50-800 mg x L(-1)) (P < 0.01); After the intervention for 24 h, the expressions of CyclinD1, CDK4 and CDK6 mRNA in all dose groups notably increased (P < 0.05), with the maximum expressions at the concentration of 200 mg x L(-1); The expression of P21 mRNA decreased, particularly at the concentration of 200 mg x L(-1) (P < 0.01).</p><p><b>CONCLUSION</b>Water extracts from Duhuo Jisheng decoction can promote chondrocyte proliferation by effecting the expression of chondrocyte G1 phase regulator mRNA.</p>
Subject(s)
Animals , Male , Rats , Cell Cycle Proteins , Genetics , Metabolism , Cell Proliferation , Cells, Cultured , Chondrocytes , Cell Biology , Metabolism , Drugs, Chinese Herbal , Pharmacology , G1 Phase , Gene Expression Regulation , RNA, Messenger , Genetics , Metabolism , Rats, Sprague-DawleyABSTRACT
<p><b>OBJECTIVE</b>The aim of this experiment is to know whether active orthopedic appliance is rationale to do presurgical treatment and seek some regularities.</p><p><b>METHODS</b>Laser scanning confocal microscopy and triple fluorochrome labeling in bone were used to observe and study detailed changes occurred in compressed suture systematically.</p><p><b>RESULTS</b>Widened suture and broken fibres between the adjoining bones was observed at the 3rd week postoperatively. At the 3rd week hyperplasia of organic tissue components was also seen. At the 6 and 9th week there were no apparent differences among all groups no matter whether in control group or experimental groups.</p><p><b>CONCLUSION</b>Retarding injury of compressed suture and surrounding tissue hyperplasia exists. With growth and development wounded suture will gradually assimilate to control group structurally and functionally.</p>